Photo of Eva C. Guinan,  MD

Eva C. Guinan, MD

Dana-Farber Cancer Institute

Dana-Farber Cancer Institute
Phone: (617) 632-4932
Fax: (617) 632-3770

Eva C. Guinan, MD

Dana-Farber Cancer Institute


  • Professor, Radiation Oncology, Harvard Medical School
  • Associate in Medicine, Hematology/Oncology, Boston Children's Hospital
  • Director, Translational Research, Radiation Oncology, Dana-Farber Cancer Institute


Research Abstract

Our research laboratory focuses on two issues: 1)overcoming problems related to allogenicity in transplantation and 2) the role of endotoxin neutralization in mitigating the toxicity of radiotherapy.

In regard to the first, allogeneic transplantation is limited by immune attacks between donor and host. Global immunosuppression has been an incomplete and highly toxic approach to this problem. Current understanding of T cell activation suggests that blocking B7-CD28 interactions of the costimulatory pathway for human T helper cells provides an innovative way of producing antigen-specific T cell hyporesponsiveness and generation of regulatory T cells. We are applying these various strategies to the transplant setting.

In regard to the second area, we have found that a human endotoxin neutralizing molecule, bacteriacidal permeability increasing protein (BPI), has the capacity to dramatically decrease the side effects of radiation. We are pursing these observations both for emergency use (such as a nuclear accident) but also in terms of effects on chemoradiotherapy of cancer.

Publications from Harvard Catalyst Profiles

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  • Mak RH, Endres MG, Paik JH, Sergeev RA, Aerts H, Williams CL, Lakhani KR, Guinan EC. Use of Crowd Innovation to Develop an Artificial Intelligence-Based Solution for Radiation Therapy Targeting. JAMA Oncol 2019. PubMed
  • Barbon CM, Janec KJ, Kelner RH, Norton JE, Guinan EC. Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation In Vitro in the Context of Costimulatory Molecule Blockade. Methods Mol Biol 2019; 1899:103-118. PubMed
  • Zhang QS, Deater M, Phan N, Marcogliese A, Major A, Guinan EC, Grompe M. Combination therapy with atorvastatin and celecoxib delays tumor formation in a Fanconi anemia mouse model. Pediatr Blood Cancer 2018. PubMed
  • Davies JK, Brennan LL, Wingard J, Cogle CR, Kapoor N, Shah AJ, Dey BR, Spitzer TR, De Lima M, Cooper LJ, Thall P, Champlin RE, Nadler LM, Guinan EC. Infusion of alloanergized donor lymphocytes after CD34-selected haploidentical myeloablative hematopoietic stem cell transplantation. Clin Cancer Res 2018. PubMed
  • Ullrich CK, Rodday AM, Bingen KM, Kupst MJ, Patel SK, Syrjala KL, Harris LL, Recklitis CJ, Chang G, Guinan EC, Terrin N, Tighiouart H, Phipps S, Parsons SK. Three sides to a story: Child, parent, and nurse perspectives on the child's experience during hematopoietic stem cell transplantation. Cancer 2017. PubMed
  • Richardson PG, Riches ML, Kernan NA, Brochstein JA, Mineishi S, Termuhlen AM, Arai S, Grupp SA, Guinan EC, Martin PL, Steinbach G, Krishnan A, Nemecek ER, Giralt S, Rodriguez T, Duerst R, Doyle J, Antin JH, Smith A, Lehmann L, Champlin R, Gillio A, Bajwa R, D'Agostino RB, Massaro J, Warren D, Miloslavsky M, Hume RL, Iacobelli M, Nejadnik B, Hannah AL, Soiffer RJ. Phase 3 trial of defibrotide for the treatment of severe veno-occlusive disease and multi-organ failure. Blood 2016; 127:1656-65. PubMed
  • Ullrich CK, Rodday AM, Bingen K, Kupst MJ, Patel SK, Syrjala KL, Harris LL, Recklitis CJ, Schwartz L, Davies S, Guinan EC, Chang G, Wolfe J, Parsons SK. Parent Outlook: How Parents View the Road Ahead as They¬†Embark on Hematopoietic Stem Cell Transplantation for¬†Their Child. Biol Blood Marrow Transplant 2015. PubMed
  • Pennarola BW, Rodday AM, Bingen K, Schwartz LA, Patel SK, Syrjala KL, Mayer DK, Ratichek SJ, Guinan EC, Kupst MJ, Hibbard JH, Parsons SK. Changing factors associated with parent activation after pediatric hematopoietic stem cell transplant. Support Care Cancer 2015. PubMed
  • Bechard LJ, Gordon C, Feldman HA, Venick R, Gura K, Guinan EC, Duggan C. Bone loss and vitamin D deficiency in children undergoing hematopoietic cell transplantation. Pediatr Blood Cancer 2015; 62:687-92. PubMed
  • Heinze KE, Rodday AM, Nolan MT, Bingen K, Kupst MJ, Patel SK, Syrjala K, Harris L, Recklitis C, Schwartz L, Davies S, Guinan EC, Noll R, Chang G, Parsons SK. The impact of pediatric blood and marrow transplant on parents: introduction of the parent impact scale. Health Qual Life Outcomes 2015; 13:46. PubMed
  • Guinan EC, Palmer CD, Mancuso CJ, Brennan L, Stoler-Barak L, Kalish LA, Suter EE, Gallington LC, Huhtelin DP, Mansilla M, Schumann RR, Murray JC, Weiss J, Levy O. Identification of single nucleotide polymorphisms in hematopoietic cell transplant patients affecting early recognition of, and response to, endotoxin. Innate Immun 2014. PubMed
  • Barbon CM, Davies JK, Voskertchian A, Kelner RH, Brennan LL, Nadler LM, Guinan EC. Alloanergization of human T cells results in expansion of alloantigen-specific CD8(+) CD28(-) suppressor cells. Am J Transplant 2014; 14:305-18. PubMed
  • DeZern AE, Guinan EC. Aplastic anemia in adolescents and young adults. Acta Haematol 2014; 132:331-9. PubMed
  • Ayas M, Saber W, Davies SM, Harris RE, Hale GA, Socie G, LeRademacher J, Thakar M, Deeg HJ, Al-Seraihy A, Battiwalla M, Camitta BM, Olsson R, Bajwa RS, Bonfim CM, Pasquini R, Macmillan ML, George B, Copelan EA, Wirk B, Al Jefri A, Fasth AL, Guinan EC, Horn BN, Lewis VA, Slavin S, Stepensky P, Bierings M, Gale RP. Allogeneic hematopoietic cell transplantation for fanconi anemia in patients with pretransplantation cytogenetic abnormalities, myelodysplastic syndrome, or acute leukemia. J Clin Oncol 2013. PubMed
  • Myers LC, Sun P, Brennan LL, London WB, Guinan EC. Effect of weight on outcomes of children undergoing hematopoietic cell transplantation. Pediatr Hematol Oncol 2013; 30:116-30. PubMed
  • Lakhani KR, Boudreau KJ, Loh PR, Backstrom L, Baldwin C, Lonstein E, Lydon M, MacCormack A, Arnaout RA, Guinan EC. Prize-based contests can provide solutions to computational biology problems. Nat Biotechnol 2013; 31:108-11. PubMed
  • Guinan EC, Agarwal S. A young adult with aplastic anemia and gray hair. Clin Chem 2013; 59:47-50. PubMed
  • Bechard LJ, Feldman HA, Venick R, Gura K, Gordon C, Sonis A, Mehta N, Guinan EC, Duggan C. Attenuation of resting energy expenditure following hematopoietic SCT in children. Bone Marrow Transplant 2012; 47:1301-6. PubMed
  • Davies JK, Barbon CM, Voskertchian A, Nadler LM, Guinan EC. Ex vivo alloanergization with belatacept: a strategy to selectively modulate alloresponses after transplantation. Cell Transplant 2012; 21:2047-61. PubMed
  • Davies JK, Barbon CM, Voskertchian AR, Nadler LM, Guinan EC. Induction of alloantigen-specific anergy in human peripheral blood mononuclear cells by alloantigen stimulation with co-stimulatory signal blockade. 2011. PubMed
  • Richardson PG, Soiffer RJ, Antin JH, Uno H, Jin Z, Kurtzberg J, Martin PL, Steinbach G, Murray KF, Vogelsang GB, Chen AR, Krishnan A, Kernan NA, Avigan DE, Spitzer TR, Shulman HM, Di Salvo DN, Revta C, Warren D, Momtaz P, Bradwin G, Wei LJ, Iacobelli M, McDonald GB, Guinan EC. Defibrotide for the treatment of severe hepatic veno-occlusive disease and multi-organ failure post stem cell transplantation: a multi-center, randomized, dose-finding trial. Biol Blood Marrow Transplant 2010; 16:1005-17. PubMed
  • Davies JK, Singh H, Huls H, Yuk D, Lee DA, Kebriaei P, Champlin RE, Nadler LM, Guinan EC, Cooper LJ. Combining CD19 redirection and alloanergization to generate tumor-specific human T cells for allogeneic cell therapy of B-cell malignancies. Cancer Res 2010; 70:3915-24. PubMed
  • Guinan EC, Hewett EK, Domaney NM, Margossian R. Outcome of hematopoietic stem cell transplant in children with congenital heart disease* Pediatr Transplant 2010. PubMed
  • Bechard LJ, Feldman HA, Gordon C, Gura K, Sonis A, Leung K, Venick R, Guinan EC, Duggan C. A multi-center, randomized, controlled trial of parenteral nutrition titrated to resting energy expenditure in children undergoing hematopoietic stem cell transplantation ("PNTREE"): rationale and design. Contemp Clin Trials 2010; 31:157-64. PubMed
  • Coppell JA, Richardson PG, Soiffer R, Martin PL, Kernan NA, Chen A, Guinan E, Vogelsang G, Krishnan A, Giralt S, Revta C, Carreau NA, Iacobelli M, Carreras E, Ruutu T, Barbui T, Antin JH, Niederwieser D. Hepatic veno-occlusive disease following stem cell transplantation: incidence, clinical course, and outcome. Biol Blood Marrow Transplant 2010; 16:157-68. PubMed
  • Davies JK, Nadler LM, Guinan EC. Expansion of allospecific regulatory T cells after anergized, mismatched bone marrow transplantation. Sci Transl Med 2009; 1:1ra3. PubMed
  • Guinan EC. Acquired aplastic anemia in childhood. Hematol Oncol Clin North Am 2009; 23:171-91. PubMed
  • Kurtzberg J,Prasad VK,Carter SL,Wagner JE,Baxter-Lowe LA,Wall D,Kapoor N,Guinan EC,Feig SA,Wagner EL,Kernan NA. Results of the Cord Blood Transplantation Study (COBLT): clinical outcomes of unrelated donor umbilical cord blood transplantation in pediatric patients with hematologic malignancies. Blood 2008; 112:4318-27. PubMed
  • Davies JK,Yuk D,Nadler LM,Guinan EC. Induction of alloanergy in human donor T cells without loss of pathogen or tumor immunity. Transplantation 2008; 86:854-64. PubMed
  • Davies JK,Gribben JG,Brennan LL,Yuk D,Nadler LM,Guinan EC. Outcome of alloanergized haploidentical bone marrow transplantation after ex vivo costimulatory blockade: results of 2 phase 1 studies. Blood 2008; 112:2232-41. PubMed
  • Duro D,Bechard LJ,Feldman HA,Klykov A,O'Leary A,Guinan EC,Duggan C. Weekly measurements accurately represent trends in resting energy expenditure in children undergoing hematopoietic stem cell transplantation. JPEN J Parenter Enteral Nutr 2008; 32:427-32. PubMed
  • Bechard LJ, Guinan EC, Feldman HA, Tang V, Duggan C. Prognostic factors in the resumption of oral dietary intake after allogeneic hematopoietic stem cell transplantation (HSCT) in children. JPEN J Parenter Enteral Nutr 2007; 31:295-301. PubMed
  • Guinan EC, Kalish LA, Berry WS, McDaniel S, Lehmann LE, Diller LR. A novel pattern of transaminase elevation associated with autologous transplant for neuroblastoma. Pediatr Transplant 2006; 10:669-76. PubMed